The bonds were placed among institutional investors and private banking clients and have a maturity of five years.
RegioJet says it is planning to invest billions of Koruna in its fleet over the next few years, including the acquisition or modernisation of around 100 coaches.
Part of the funds raised through the bond issue will be used to refinance existing debt.
According to its preliminary first quarter results, RegioJet generated a gross profit of Koruna 18m ($US 790,000) in the first quarter, with adjusted Ebitda reaching Koruna 87.5m.
Passenger numbers on long-distance services increased 12.6% compared with the first quarter of 2018, reaching 1.4 million. First quarter revenues increased 10% year-on-year to Koruna 330m.