The line is primarily used by Go Transit commuter services, although CN will retain running rights for freight trains following the transfer of ownership.
"Ontario is taking the next step towards two-way, all-day Regional Express Rail service along the Kitchener line," says Metrolinx president and CEO Mr Bruce McCuaig. "Ownership and control of our infrastructure is key to improving the existing service and planning for even more service on this line in the future."
Metrolinx has also acquired land in Kitchener for future train stabling facilities.
Go Transit will double its Kitchener - Toronto services in 2016.