Starting at San Marco Square in the city centre, Line 2 will run to the city's main station, Santa Maria Novella, where it will interchange with Line 1 and the future Line 3, before continuing west to serve Florence Belfiore high-speed station and Peretola Airport.

The project is being implemented by a consortium comprising Consorzio Cooperative Costruzioni, Consorzio Toscano Costruzioni, DiCos, Ciet Impianti, AnsaldoBreda, Ansaldo STS, Alstom, Sirti, and Grandi Lavori Fincosit.

Alstom's share of the contract includes track, electrification, substations, and power supplies.

The line is part of a broader project to construct three new lines by 2017, partly utilising former heavy rail alignments.

AnsaldoBreda will supply 29 five-section bidirectional Siro low-floor LRVs for lines 2 and 3 under a €60m contract signed in June 2005.

The city's first light rail line opened in February 2010.